Microinjection of rabbit hemoglobin messenger RNA into amphibian oocytes and embryos.
نویسندگان
چکیده
After microinjection of hemoglobin mRNA from rabbit reticulocytes (9S mRNA) into Xenopus laevis oocytes, the cytological structure remains normal. The peroxidase reaction does not show an accumulation of synthesized hemoglobin in the germinal vesicle. Fertilized Pleurodeles Waltlii eggs synthesize rabbit hemoglobin after injection of 9S mRNA; hemoglobin synthesis decreases as development progresses. Micromeres and macromeres synthesize equal amounts of hemoglobin, despite the fact that the micromeres contain many more ribosomes. Descendants of injected blastomeres divide more slowly and do not differentiate; they ultimately undergo cytolysis.
منابع مشابه
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A cell-free protein-synthesizing system derived from rabbit reticulocytes is described which is dependent on the addition of transfer RNA for the translation of endogenous hemoglobin messenger RNA. Product analysis indicates that the system is active in the initiation of new chains. When hemoglobin is synthesized in the presence of a limiting amount of tRNA, there is a 50% decrease in (Y chain ...
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ورودعنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 70 2 شماره
صفحات -
تاریخ انتشار 1973